GCN5L2 (C26A10) Rabbit mAb #3305
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 94 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
GCN5L2 (C26A10) Rabbit mAb detects endogenous levels of total GCN5L2 protein. The antibody does not cross-react with the related PCAF protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Bovine, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human GCN5L2.
Background
General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) is a transcription adaptor protein and a histone acetyltransferase (HAT) that functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (1). GCN5L2 is 73% homologous to the p300/CBP-associated factor PCAF, another HAT protein found in similar complexes (2). Free GCN5L2 acetylates histone H3 on Lys14; however, when part of coactivator complexes, GCN5L2 acetylates histone H3 at Lys9, 14, 18, and 23, and to a smaller extent histones H4 and H2B (3). Histone acetylation contributes to gene activation by modulating chromatin structure and recruiting additional coactivator proteins that contain acetyl-lysine binding bromodomains (4). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (5,6), transcription co-activators (PGC1-α) (7), and nuclear receptors (Steroidogenic Factor 1) (8). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (5-8). GCN5L2 is recruited to gene promoters during transactivation through interactions with multiple transcription activator proteins such as Myc, E2F, p53, and BRCA1 (9-12). The STAGA and TFTC complexes also interact with SAP130 and DDB1, two structurally related proteins involved in RNA splicing and DNA repair, suggesting roles for GCN5L2 in processes other than transcription activation (13).
- Candau, R. et al. (1996) Mol Cell Biol 16, 593-602.
- Yang, X.J. et al. (1996) Nature 382, 319-24.
- Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.
- Yang, X.J. (2004) Bioessays 26, 1076-87.
- Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.
- Tomita, A. et al. (2000) Oncogene 19, 444-51.
- Lerin, C. et al. (2006) Cell Metab 3, 429-38.
- Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.
- Liu, X. et al. (2003) J Biol Chem 278, 20405-12.
- Lang, S.E. et al. (2001) J Biol Chem 276, 32627-34.
- Candau, R. et al. (1997) Oncogene 15, 807-16.
- Oishi, H. et al. (2006) J Biol Chem 281, 20-6.
- Brand, M. et al. (2001) EMBO J 20, 3187-96.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.